The crystal structure of aThermoactinomyces vulgariscyclo⁄maltodextrin-binding protein (TvuCMBP) complexed withc-cyclodextrin has been deter-mined. LikeEscherichia colimaltodextrin-binding protein (EcoMBP) and other bacterial sugar-binding proteins,TvuCMBP consists of two domains, an N- and a C-domain, both of which are composed of a centralb-sheet surrounded bya-helices; the domains are joined by a hinge region contain-ing three segments.