Opportunistic infections are infections that occur due to a decrease in the immune system and can cause serious conditions in people with weak immune systems such as in people with HIV / AIDS. Cryptosporidium sp. and Giardia lamblia are examples of the opportunistic intestinal protozoa that can cause chronic diarrhea so that it becomes dehydrated and malnutrition which can increase mortality and morbidity in HIV sufferers. Observational study with cross-sectional design on 50 fecal samples of HIV / AIDS patients. Cryptosporidium sp. and Giardia lamblia infections tested microscopically using Ziehl Neelsen's staining and molecular examination with DNA isolation,and looking at the relationship between CD4 cell counts and history of diarrhea with Cryptosporidium Giardia lamblia infections. The results obtained 2 respondents (4%) positive infected with Cryptosporidium sp., There is a significant relationship between CD4 cell counts with Cryptosporidium sp. infection, p-value= () and there is a significant relationship between Giardia lamblia infections with a history of diarrhea, p-value= (p | The relationship of CD4 cells and history of diarrhea to cryptosporidium sp. and giardia lamblia infections in HIV/AIDS patients in west sumatra indonesia and its DNA isolation
