Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Critical Care giúp cho các bạn có thêm kiến thức về ngành y học đề tài: Biotransformation of ginsenosides Rb1, Rg3 and Rh2 in rat gastrointestinal tracts. | Qian and Cai Chinese Medicine 2010 5 19 http content 5 1 19 CHINESE MEDICINE RESEARCH Open Access Biotransformation of ginsenosides Rb1 Rg3 and Rh2 in rat gastrointestinal tracts Tianxiu Qian 1 2 and Zongwei Cai 1 Abstract Background Ginsenosides such as Rb1 Rg3 and Rh2 are major bioactive components of Panax ginseng. This in vivo study investigates the metabolic pathways of ginsenosides Rb1 Rg3 and Rh2 orally administered to rats. Methods High performance liquid chromatography-mass spectrometry LC-MS and tandem mass spectrometry MS-MS techniques particularly liquid chromatography electrospray ionization mass spectrometry LC-ESI-MS were used to identify the metabolites. Results Six metabolites of Rb1 six metabolites of Rg3 and three metabolites of Rh2 were detected in the feces samples of the rats. Rh2 was a metabolite of Rb1 and Rg3 whereas Rg3 was a metabolite of Rb1. Some metabolites such as protopanaxadiol and monooxygenated protopanaxadiol are metabolites of all three ginsenosides. Conclusion Oxygenation and deglycosylation are two major metabolic pathways of the ginsenosides in rat gastrointestinal tracts. Background Panax ginseng Renshen is used in Chinese medicines to treat various conditions such as debility ageing stress diabetes insomnia and sexual inadequacy 1-3 . The major bioactive components of P ginseng are O-glyco-sides of the triterpen dammarane saponins known as gin-senosides 4 5 which exhibit properties such as antiinflammation and anti-tumor 6-8 . Over 80 ginsenosides have been isolated from P ginseng 9 . Rb1 Rg3 and Rh2 are three major ginsenosides with various bioactivities. Rb1 which is the most abundant among all ginsenosides 5 protects against free radical damage maintains normal cholesterol and blood pressure 10 and inhibits the induction phase of long-term potentiation by high frequency stimulation in the dentate gyrus of the brain 11 . Rb1 also rescues hippocampal neurons from lethal ischemic damage 12 and .
