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Báo cáo y học: "Small interfering RNA mediated Poly (ADP-ribose) Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Small interfering RNA mediated Poly (ADP-ribose) Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line. | Aneja et al. Journal of Inflammation 2011 8 3 http www.journal-inflammation.eom content 8 1 3 JOURNAL OF INFLAMMATION RESEARCH Open Access Small interfering RNA mediated Poly ADP-ribose Polymerase-1 inhibition upregulates the heat shock response in a murine fibroblast cell line p ỉdch I A nd1UnnCinrlin2 II ill . ctofiai 3 R .icili .i Vinfmmlli4 Pl eol I fìũl mid3 Rdjesh K Anejd ndlllld Sjodln Julld V Gefter Bdsllld Zlngdrelll Russell L Delude Abstract Poly ADP-rlbose polymerdse-1 PARP-1 Is d highly conserved multlfunctlondl enzyme dnd Its cdtdlytlc dctivity Is stlmuldted by DNA bredks. The dctlvdtlon of PARP-1 dnd subsequent depletion of nlcotlndmlde ddenlne dlnucleotlde NAD dnd ddenoslne trlphosphdte ATP contrlbutes to slgnlflcdnt cytotoxlclty ln lnfldmmdtlon of vdrlous etlologles. On the contrary lnductlon of hedt shock response dnd production of hedt shock proteln 70 HSP-70 ls d cytoprotectlve defense mechdnlsm ln lnfldmmdtlon. Recent ddtd suggests thdt PARP-1 moduldtes the expresslon of d number of celluldr protelns dt the trdnscrlptlondl level. In thls study smdll lnterferlng RNA slRNA medldted PARP-1 knockdown ln murlne wlld-type flbrobldsts dugmented hedt shock response ds compdred to untredted cells ds evdludted by qudntltdtlve dndlysls of HSP-70 mRNA dnd HSP-70 proteln expresslon . These events were dssocldted wlth lncredsed DNA blndlng of the hedt shock fdctor-1 HSF-1 the mdjor trdnscrlptlon fdctor of the hedt shock response. Co-lmmunopreclpltdtlon experlments ln nucledr extracts of the wlld type cells demonstrdted thdt PARP-1dlrectly lnterdcted wlth HSF-1. These ddtd demonstrdte thdt ln wlld type flbrobldsts PARP-1 pldys d plvotdl role ln moduldtlng the hedt shock response both through dlrect lnterdctlon wlth HSF-1 dnd poly ADP-rlbosyldtlon . Introduction Poly ADP-ribose polymerase-1 PARP-1 is a highly conserved chromatin bound enzyme 1 2 and plays an important role in DNA repair gene transcription cellcycle progression cell death and maintenance of .