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Báo cáo y học: "Novel anti-inflammatory role of SLPI in adipose tissue and its regulation by high fat diet"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Novel anti-inflammatory role of SLPI in adipose tissue and its regulation by high fat diet. | Adapala et al. Journal of Inflammation 2011 8 5 http www.journal-inflammation.eom content 8 1 5 JOURNAL OF INFLAMMATION RESEARCH Open Access Novel anti-inflammatory role of SLPI in adipose tissue and its regulation by high fat diet Venkata J Adapala1 Kimberly K Buhman2 Kolapo M Ajuwon1 Abstract Background Secretory leucocyte protease inhibitor SLPI is an anti-inflammatory protein that is constitutively expressed in multiple cell types where it functions to counteract localized tissue inflammation by its antiinflammatory antimicrobial and anti-protease properties. Little is known about the expression and implication of SLPI in the regulation of adipose tissue inflammation. Therefore we tested the hypothesis that obesity induces expression of SLPI in adipose tissue where it functions to counteract adipocyte inflammation. Methods Male C57BL6 mice were fed a high fat 60 fat calories or a control diet 10 fat calories diet for 12 weeks. Adipose tissue expression of SLPI was determined by western blotting and PCR. Fully differentiated adipocytes 3T3-L1 were treated with lipopolysaccharide LPS 100 ng ml or peptidoglycan 10 pg ml for 24 hours in the presence or absence of SLPI. Media was collected for interleukin 6 IL-6 analysis by enzyme-linked immune absorbent assay ELISA . RNA was isolated for gene expression analysis by real-time polymerase chain reaction RT-PCR . Results Visceral fat mesenteric and epididymal express a higher level of SLPI than subcutaneous fat. The expression of SLPI is mostly in the stromal vascular fraction compared to adipocytes. We also confirmed in vitro that activation of TLR2 and 4 with peptidoglycan and LPS respectively leads to induction of SLPI. Finally we confirmed that SLPI exerted an anti-inflammatory effect in adipocytes treated with LPS by causing a reduction in expression of IL-6 via a mechanism that included stabilization of cellular IKBa expression. Conclusion Our results show that SLPI is also expressed in adipocytes and adipose .