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Báo cáo y học: " Early A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Early A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity. | Journal of Inflammation BioMed Central Research A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity Catriona M Turnbull1 Danny McClure1 Adriano G Rossi2 and Ian L Megson 3 Open Access Address 1Centre for Cardiovascular Science Queen s Medical Research Institute University of Edinburgh Edinburgh UK 2MRC Centre for Inflammation Research Queen s Medical Research Institute University of Edinburgh Edinburgh UK and 3Free Radical Research Facility UHI Millennium Institute Inverness UK Email Catriona M Turnbull - catriona.scott@ed.ac.uk Danny McClure - d.mcclure@ed.ac.uk Adriano G Rossi - a.g.rossi@ed.ac.uk Ian LMegson - ian.megson@uhi.ac.uk Corresponding author Published 28 September 2006 Received 21 April 2006 Accepted 28 September 2006 Journal of Inflammation 2006 3 12 doi 10.1186 1476-9255-3-12 This article is available from http www.journal-inflammation.cOm content 3 1 12 2006 Turnbull et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract__ Background Prostaglandin H2 synthase PGHS is the enzyme that catalyses the two-stage conversion of arachidonic acid to prostaglandin H2 PGH2 prior to formation of prostanoids that are important in inflammation. PGHS isozymes -1 and -2 are the target for nonsteroidal antiinflammatory drugs NSAIDs . Given the rekindled interest in specific anti-inflammatory PGHS inhibitors with reduced unwanted side effects it is of paramount importance that there are reliable and efficient techniques to test new inhibitors. Here we describe a novel in vitro electron paramagnetic resonance EPR -based assay for measuring the activity of PGHS-1. Methods We validated a novel in vitro