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báo cáo khoa học: "The impact of Ty3-gypsy group LTR retrotransposons Fatima on B-genome specificity of polyploid wheats"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The impact of Ty3-gypsy group LTR retrotransposons Fatima on B-genome specificity of polyploid wheats | Salina et al. BMC Plant Biology 2011 11 99 http www.biomedcentral.com 1471-2229 11 99 BMC Plant Biology RESEARCH ARTICLE Open Access The impact of Ty3-gypsy group LTR retrotransposons Fatima on B-genome specificity of polyploid wheats 1 1 1 12 2 Elena A Salina Ekaterina M Sergeeva Irina G Adonina Andrey B Shcherban Harry Belcram Cecile Huneau and Boulos Chalhoub2 Abstract Background Transposable elements TEs are a rapidly evolving fraction of the eukaryotic genomes and the main contributors to genome plasticity and divergence. Recently occupation of the A- and D-genomes of allopolyploid wheat by specific TE families was demonstrated. Here we investigated the impact of the well-represented family of gypsy LTR-retrotransposons Fatima on B-genome divergence of allopolyploid wheat using the fluorescent in situ hybridisation FISH method and phylogenetic analysis. Results FISH analysis of a BAC clone BAC_2383A24 initially screened with Spelt1 repeats demonstrated its predominant localisation to chromosomes of the B-genome and its putative diploid progenitor Aegilops speltoides in hexaploid genomic formula BBAADD and tetraploid genomic formula BBAA wheats as well as their diploid progenitors. Analysis of the complete BAC_2383A24 nucleotide sequence 113 605 bp demonstrated that it contains 55.6 TEs 0.9 subtelomeric tandem repeats Spelt1 and five genes. LTR retrotransposons are predominant representing 50.7 of the total nucleotide sequence. Three elements of the gypsy LTR retrotransposon family Fatima make up 47.2 of all the LTR retrotransposons in this BAC. In situ hybridisation of the Fatima_2383A24-3 subclone suggests that individual representatives of the Fatima family contribute to the majority of the B-genome specific FISH pattern for BAC_2383A24. Phylogenetic analysis of various Fatima elements available from databases in combination with the data on their insertion dates demonstrated that the Fatima elements fall into several groups. One of these groups containing .