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báo cáo khoa học: " Mapping QTLs for oil traits and eQTLs for oleosin genes in jatropha"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Mapping QTLs for oil traits and eQTLs for oleosin genes in jatropha | Liu et al. BMC Plant Biology 2011 11 132 http www.biomedcentral.com 1471-2229 11 132 BMC Plant Biology RESEARCH ARTICLE Open Access Mapping QTLs for oil traits and eQTLs for oleosin genes in jatropha Peng Liu Chun Ming Wang Lei Li Fei Sun Peng Liu and Gen Hua Yue Abstract Background The major fatty acids in seed oil of jatropha a biofuel crop are palmitic acid C16 0 stearic acid C18 0 oleic acid C18 1 and linoleic acid C18 2 . High oleic acid and total oil content are desirable for jatropha breeding. Until now little was known about the genetic bases of these oil traits in jatropha. In this study quantitative trait locus QTL and expression QTL analyses were applied to identify genetic factors that are relevant to seed oil traits in jatropha. Results Composite interval mapping identified 18 QTL underlying the oil traits. A highly significant QTL qC18 1-1 was detected at one end of linkage group LG 1 with logarithm of the odd LOD 18.4 and percentage of variance explained PVE 36.0 . Interestingly the QTL qC18 1-1 overlapped with qC18 2-1 controlling oleic acid and linoleic acid compositions. Among the significant QTL controlling total oil content qOilC-4 was mapped on LG4 a relatively high significant level with LOD 5.0 and PVE 11.1 . Meanwhile oleosins are the major composition in oil body affecting oil traits we therefore developed SNP markers in three oleosin genes Olel Olell and Olelll which were mapped onto the linkage map. Olel and Olelll were mapped on LG5 closing to QTLs controlling oleic acid and stearic acid. We further determined the expressions of Olel Olell and Olelll in mature seeds from the QTL mapping population and detected expression QTLs eQTLs of the three genes on LGs 5 6 and 8 respectively. The eQTL of Olelll qOlelll-5 was detected on LG5 with PVE 11.7 and overlapped with QTLs controlling stearic acid and oleic acid implying a cis- or trans-element for the Olelll affecting fatty acid compositions. Conclusion We identified 18 QTLs underlying the .