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báo cáo khoa học: " The influence of bisphosphonates on human osteoblast migration and integrin aVb3/tenascin C gene expression in vitro"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The influence of bisphosphonates on human osteoblast migration and integrin aVb3/tenascin C gene expression in vitro | Koch et al. Head Face Medicine 2011 7 4 http www.head-face-med.eom content 7 1 4 HEAD FACE MEDICINE RESEARCH Open Access The influence of bisphosphonates on human osteoblast migration and integrin aVb3 tenascin C gene expression in vitro 1 1111 2 Felix P Koch Annette Wunsch Christina Merkel Thomas Ziebart Andreas Pabst Sareh Said Yekta Marco Blessmann 2 3 Ralf Smeets3 Abstract Background Bisphosphonates are therapeutics of bone diseases such as Paget s disease multiple myeloma or osteoclastic metastases. As a severe side effect the bisphosphonate induced osteonecrosis of the jaw BONJ often requires surgical treatment and is accompanied with a disturbed wound healing. Therefore the influence on adhesion and migration of human osteoblasts hOB after bisphosphonate therapy has been investigated by morphologic as well as gene expression methods. Methods By a scratch wound experiment which measures the reduction of defined cell layer gap the morphology and migration ability of hOB was evaluated. A test group of hOB which was stimulated by zoledronate 5 X 10-5M and a control group of unstimulated hOB were applied. Furthermore the gene expression of integrin aVb3 and tenascin C was quantified by Real-Time rtPCR at 5data points over an experimental period of 14 days. The bisphosphonates zoledronate ibandronate and clodronate have been compared with an unstimulated hOB control. Results After initially identical migration and adhesion characteristics zoledronate inhibited hOB migration after 50 h of stimulation. The integrinavb3 and tenascin C gene expression was effected by bisphosphonates in a cell line dependent manner with decreased respectively inconsistent gene expression levels over time. The nonnitrogen containing bisphosphonates clodronate led to decreased gene expression levels. Conclusion Bisphosphonates seem to inhibit hOB adhesion and migration. The integrin aVb3 and tenascin C gene expression seem to be dependent on the cell line. BONJ could be enhanced by an .