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Báo cáo y học: " Complete coding sequence characterization and comparative analysis of the putati"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Complete coding sequence characterization and comparative analysis of the putati | Linsuwanon et al. Virology Journal 2011 8 5 http www.virologyj.eom content 8 1 5 J VIROLOGY JOURNAL RESEARCH Open Access Complete coding sequence characterization and comparative analysis of the putative novel human rhinovirus HRV species C and B Piyada Linsuwanon1 Sunchai Payungporn2 Kamol Suwannakarn1 Thaweesak Chieochansin1 Apiradee Theamboonlers1 Yong Poovorawan1 Abstract Background Human Rhinoviruses HRVs are well recognized viral pathogens associated with acute respiratory tract illnesses RTIs abundant worldwide. Although recent studies have phylogenetically identified the new HRV species HRV-C data on molecular epidemiology genetic diversity and clinical manifestation have been limited. Result To gain new insight into HRV genetic diversity we determined the complete coding sequences of putative new members of HRV species C HRV-CU072 with 1 prevalence and HRV-B HRV-CU211 identified from clinical specimens collected from pediatric patients diagnosed with a symptom of acute lower RTI. Complete coding sequence and phylogenetic analysis revealed that the HRV-CU072 strain shared a recent common ancestor with most closely related Chinese strain N4 . Comparative analysis at the protein level showed that HRV-CU072 might accumulate substitutional mutations in structural proteins as well as nonstructural proteins 3C and 3 D. Comparative analysis of all available HRVs and HEVs indicated that HRV-C contains a relatively high G C content and is more closely related to HEV-D. This might be correlated to their replication and capability to adapt to the high temperature environment of the human lower respiratory tract. We herein report an infrequently occurring intra-species recombination event in HRV-B species HRV-CU211 with a crossing over having taken place at the boundary of VP2 and VP3 genes. Moreover we observed phylogenetic compatibility in all HRV species and suggest that dynamic mechanisms for HRV evolution seem to be related to recombination events. These findings