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Báo cáo y học: "Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine"

Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O/Fujian/CHA/5/99 isolated from swine | Bai et al. Virology Journal 2010 7 208 http www.virologyj.eom content 7 1 208 VIROLOGY JOURNAL RESEARCH Open Access Genetic characterization of the cell-adapted PanAsia strain of foot-and-mouth disease virus O Fujian CHA 5 99 isolated from swine XingWen Bai HuiFang Bao PingHua Li Pu Sun WenDong Kuang YiMei Cao ZengJun Lu ZaiXin Liu XiangTao Liu Abstract Background According to Office International Des Epizooties OIE Bulletin the PanAsia strain of Foot-and-Mouth Disease Virus FMDV was invaded into the People s Republic of China in May 1999. It was confirmed that the outbreaks occurred in Tibet Hainan and Fujian provinces. In total 1280 susceptible animals 68 cattle 1212 swine were destroyed for the epidemic control. To investigate the distinct biological properties we performed plaque assay estimated the pathogenicity in suckling mice and determined the complete genomic sequence of FMDV swine-isolated O Fujian CHA 5 99 strain. In addition a molecular modeling was carried out with the external capsid proteins. Results The pathogenicity study showed that O Fujian CHA 5 99 had high virulence with respect to infection in 3-day-old suckling-mice LD50 10-8.3 compared to O Tibet CHA 1 99 LD50 10-7.0 which isolated from bovine. The plaque assay was distinguishable between O Fujian CHA 5 99 and O Tibet CHA 1 99 by their plaque phenotypes. O Fujian CHA 5 99 formed large plaque while O Tibet CHA 1 99 formed small plaque. The 8 172 nucleotides nt of O Fujian CHA 5 99 was sequenced and a phylogenetic tree was generated from the complete nucleotide sequences of VP1 compared with other FMDV reference strains. The identity data showed that O Fujian CHA 5 99 is closely related to O AS SKR 2002 94.1 similarity . Based on multiple sequence alignments comparison of sequences showed that the characteristic nucleotide amino acid mutations were found in the whole genome of O Fujian CHA 5 99. Conclusion Our finding suggested that C275T substitution in IRES of O Fujian CHA 5 99 may induce .