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Báo cáo y học: "Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2 | Sun et al. Virology Journal 2010 7 274 http www.virologyj.eom content 7 1 274 VIROLOGY JOURNAL RESEARCH Open Access Development and validation of an ELISA using a protein encoded by ORF2 antigenic domain of porcine circovirus type 2 Shi-Qi Sun Hui-Chen Guo De-Hui Sun Shuang-Hui Yin You-Jun Shang Xue-Peng Cai Xiang-Tao Liu Abstract Background The capsid protein ORF2 is a major structural protein of porcine circovirus type 2 PCV2 . A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here we reported an indirect enzyme-linked immunosorbent assay I-ELISA by using an antigenic domain 113-147AA of ORF2-encoded antigen expressed in E. coli for diagnosis of PCV infection. Results The ELISA was performed on 288 serum samples collected from different porcine herds and compared with an indirect immunofluorescent assay IFA . In total 262 of 288 samples were positive as indicated by both I-ELISA and IFA. The specificity and sensitivity of I-ELISA were 87.7 and 93.57 . Conclusions This ELISA is suitable for detection and discrimination of PCV2 infection in both SPF and farm antisera. Background Porcine circovirus PCV is a member of circoviridae. It is a small non-enveloped DNA virus with a circular single-stranded genome 1 . Genomic analysis revealed that there are two distinct genotypes of PCV 2-5 . The PCV1 was identified as a persistent non-cytopathic contaminant of the porcine kidney cell line PK-15 6 7 . In contrast PCV2 is considered the primary causative agent for post weaning multisystemic wasting syndrome PMWS 8-11 . The genome DNA of both PCV1 and PCV2 consist of several major open reading frames of these ORF1 ORF2 and ORF3 have been studied. The ORF1 encodes a replication-associated protein of 35.7 kDa 12 while ORF2 encodes a major immunogenic capsid protein of approximately 30 kDa 13 and ORF3 plays a major role in PCV2-induced .