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báo cáo khoa học: " A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica | Yoshida et al. BMC Plant Biology 2010 10 55 http www.biomedcentral.com 1471-2229 10 55 BMC Plant Biology RESEARCH ARTICLE _ Open Access A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed Striga hermonthica Satoko Yoshida 1 Juliane K Ishida1 2 Nasrein M Kamal3 Abdelbagi M Ali3 Shigetou Namba2 and Ken Shirasu 1 Abstract Background The obligate parasitic plant witchweed Strigahermonthica infects major cereal crops such as sorghum maize and millet and is the most devastating weed pest in Africa. An understanding of the nature of its parasitism would contribute to the development of more sophisticated management methods. However the molecular and genomic resources currently available for the study of S. hermonthica are limited. Results We constructed a full-length enriched cDNA library of S. hermonthica sequenced 37 710 clones from the library and obtained 67 814 expressed sequence tag EST sequences. The ESTs were assembled into 17 317 unigenes that included 10 319 contigs and 6 818 singletons. The S. hermonthica unigene dataset was subjected to a comparative analysis with other plant genomes or ESTs. Approximately 80 of the unigenes have homologs in other dicotyledonous plants including Arabidopsis poplar and grape. We found that 589 unigenes are conserved in the hemiparasitic Triphysaria species but not in other plant species. These are good candidates for genes specifically involved in plant parasitism. Furthermore we found 1 445 putative simple sequence repeats SSRs in the S. hermonthica unigene dataset. We tested 64 pairs of PCR primers flanking the SSRs to develop genetic markers for the detection of polymorphisms. Most primer sets amplified polymorphicbands from individual plants collected at a single location indicating high genetic diversity in S. hermonthica. We selected 10 primer pairs to analyze S. hermonthica harvested in the field from different host species and geographic locations. A clustering analysis suggests