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báo cáo khoa học: " A rich TILLING resource for studying gene function in Brassica rapa"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: A rich TILLING resource for studying gene function in Brassica rapa | Stephenson et al. BMC Plant Biology 2010 10 62 http www.biomedcentral.com 1471-2229 10 62 BMC Plant Biology METHODOLOGY ARTICLE Open Access A rich TILLING resource for studying gene function in Brassica rapa 1 2 1 1 3 3 Pauline Stephenson David Baker Thomas Girin Amandine Perez Stephen Amoah Graham J King Lars 0stergaard1 Abstract Background The Brassicaceae family includes the model plant Arabidopsis thaliana as well as a number of agronomically important species such as oilseed crops in particular Brassica napus B. juncea and B. rapa and vegetables eg. B. rapa and B. oleracea . Separated by only 10-20 million years Brassica species and Arabidopsis thaliana are closely related and it is expected that knowledge obtained relating to Arabidopsis growth and development can be translated into Brassicas for crop improvement. Moreover certain aspects of plant development are sufficiently different between Brassica and Arabidopsis to warrant studies to be carried out directly in the crop species. However mutating individual genes in the amphidiploid Brassicas such as B. napus and B. juncea may on the other hand not give rise to expected phenotypes as the genomes of these species can contain up to six orthologues per single-copy Arabi-dopsis gene. In order to elucidate and possibly exploit the function of redundant genes for oilseed rape crop improvement it may therefore be more efficient to study the effects in one of the diploid Brassica species such as B. rapa. Moreover the ongoing sequencing of the B. rapa genome makes this species a highly attractive model for Brassica research and genetic resource development. Results Seeds from the diploid Brassica A genome species B. rapa were treated with ethyl methane sulfonate EMS to produce a TILLING Targeting Induced Local Lesions In Genomes population for reverse genetics studies. We used the B. rapa genotype R-o-18 which has a similar developmental ontogeny to an oilseed rape crop. Hence this resource is expected to be well