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Báo cáo khoa học: " An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction | Virology Journal BioMed Central Research An analysis of the subtypes of dengue fever infections in Barbados 2003-2007 by reverse transcriptase polymerase chain reaction M Gittens-St Hilaire 1 2 and Nicole Clarke-Greenidge2 Address University of the West Indies Faculty of Medical Sciences Queen Elizabeth Hospital Martindales Road St. Michael Barbados and 2Leptospira Laboratory Ministry of Health Enmore 2 Lower Collymore Rock St. Michael Barbados Email M Gittens-St Hilaire - margitts@gmail.com Nicole Clarke-Greenidge - desanic2001@yahoo.co.uk Corresponding author Open Access Published 17 December 2008 Received 7 August 2008 _iaaao r.i -A J.IA I loAiiTAt A- - v r Id Accepted 17 December 2008 Virology Journal 2008 5 152 doi l0.ll86 l743-422X-5-l52 This article is available from http www.virologyj.cOm content 5 l l52 2008 Gittens-St Hilaire and Greenidge licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background To perform a retrospective analysis of patients with IgM antibodies to dengue fever infection to determine the serotypes present by molecular techniques. A representative sample 20 per year of patients diagnosed with dengue fever infection were selected based on the detection of IgM antibodies in the acute phase serum sample. RNA was extracted from each sample and reverse transcribed. Following this the amplicons were electrophoresed and serotyped based on band sizes. Results This study consisted of 7l males and l0l females ranging in age from 0 - 50 yrs giving a total of l72 persons with an average of 34.4 patients per year. Onset averaged 6.9 days ranging from 0-90 days. Common symptoms were as follows fever 69 .