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Báo cáo hóa học: "Prognostic impact of clinical course-specific mRNA expression profiles in the serum of perioperative patients with esophageal cancer in the ICU: a case control study"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Prognostic impact of clinical course-specific mRNA expression profiles in the serum of perioperative patients with esophageal cancer in the ICU: a case control study | Takahashi et al. Journal of Translational Medicine 2010 8 103 http www.translational-medicine.eom content 8 1 103 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Prognostic impact of clinical course-specific mRNA expression profiles in the serum of perioperative patients with esophageal cancer in the ICU a case control study 1.2 2 1.2 2 2 Shunsaku Takahashi 1 Norimasa Miura Tomomi Harada 1 ZhongZhi Wang Xinhui Wang I_I I A-J . AA 1 11 f I I c I 1r Vi I . 3 f- I-- I -A U l rs r I I AVA -A 1 4 11 I A A I l A I I_I r Á Al A -A 2 7S . r l A I AVA I IA A A S -A Iz I 1 r tr r l A I c l A I A 5 Hideyuki isuookura Yoshiaki oshima Junichi Hasegawa Yoshimi Inagaki Goshi Shiota Abstract Background We previously reported that measuring circulating serum mRNAs using quantitative one-step realtime RT-PCR was clinically useful for detecting malignancies and determining prognosis. The aim of our study was to find crucial serum mRNA biomarkers in esophageal cancer that would provide prognostic information for postesophagectomy patients in the critical care setting. Methods We measured serum mRNA levels of 11 inflammatory-related genes in 27 post-esophagectomy patients admitted to the intensive care unit ICU . We tracked these levels chronologically perioperatively and postoperatively until the two-week mark investigating their clinical and prognostic significance as compared with clinical parameters. Furthermore we investigated whether gene expression can accurately predict clinical outcome and prognosis. Results Circulating mRNAs in postoperative esophagectomy patients had gene-specific expression profiles that varied with the clinical phase of their treatment. Multivariate regression analysis showed that upregulation of IL-6 VWF and TGF-P1 mRNA in the intraoperative phase p 0.016 0.0021 and 0.009 and NAMPT and MUC1 mRNA on postoperative day 3 p 0.01 were independent factors of mortality in the first year of follow-up. Duration of ventilator dependence DVD and ICU stay were