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Báo cáo sinh học: " Localization of deformed wing virus infection in queen and drone Apis mellifera L"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Localization of deformed wing virus infection in queen and drone Apis mellifera L | Virology Journal BioMed Central Short report Open Access Localization of deformed wing virus infection in queen and drone Apis mellifera L Julie Fievet1 Diana Tentcheva1 Laurent Gauthier 1 Joachim de Miranda2 Francois Cousserans1 Marc Edouard Colin1 and Max Bergoin1 Address 1Laboratoire de Pathologie Comparée des Invertébrés EPHE UMR 1231 Biologie Intégrative et Virologie des Insectes INRA Université Montpellier II Place Bataillon 34095 Montpellier France and 2Department of Entomology Penn State University PA16802 USA Email Julie Fievet - julie.fievet@ifremer.fr DianaTentcheva - dianatent@yahoo.fr Laurent Gauthier - gauthier@univ-montp2.fr Joachim de Miranda - joachimdemiranda@yahoo.com Francois Cousserans - coussera@ensam.inra.fr Marc Edouard Colin - colinme@ensam.inra.fr Max Bergoin - bergoin@ensam.inra.fr Corresponding author Published 28 March 2006 Virology Journal 2006 3 16 doi 10.1186 1743-422X-3-16 Received 14 November 2005 Accepted 28 March 2006 This article is available from http www.virologyj.cOm content 3 1 16 2006 Fievet et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract The distribution of deformed wing virus infection within the honey bee reproductive castes queens drones was investigated by in situ hybridization and immunohistology from paraffin embedded sections. Digoxygenin or CY5.5 fluorochrome end-labelled nucleotide probes hybridizing to the 3 portion of the DWV genome were used to identify DWV RNA while a monospecific antibody to the DWV-VP1 structural protein was used to identify viral proteins and particles. The histological data were confirmed by quantitative RT-PCR of dissected organs. Results showed that DWV infection is not restricted to the digestive tract of the bee but spread in the