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Báo cáo hóa học: " Detection and frequency of recombination in tomato-infecting begomoviruses of South and Southeast Asia"

Tham khảo tài liệu 'báo cáo hóa học: " detection and frequency of recombination in tomato-infecting begomoviruses of south and southeast asia"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Virology Journal BioMed Central Research Open Access Detection and frequency of recombination in tomato-infecting begomoviruses of South and Southeast Asia HC Prasanna and Mathura Rai Address Indian Institute of Vegetable Research P B 5002 P 0-B H U Varanasi Uttar Pradesh 221005 India Email HC Prasanna - prasanahc@yahoo.com Mathura Rai - mathura.rai@gmail.com Corresponding author Published 26 October 2007 Received 15 September 2007 Accepted 26 October 2007 Virology Journal 2007 4 111 doi l0.ll86 I743-422X-4-III This article is available from http www.virologyj.com content 4 1 1 11 2007 Prasanna and Rai licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Tomato-infecting begomoviruses are widely distributed across the world and cause diseases of high economic impact on wide range of agriculturally important crops. Though recombination plays a pivotal role in diversification and evolution of these viruses it is currently unknown whether there are differences in the number and quality of recombination events amongst different tomato-infecting begomovirus species. To examine this we sought to characterize the recombination events estimate the frequency of recombination and map recombination hotspots in tomato-infecting begomoviruses of South and Southeast Asia. Results Different methods used for recombination breakpoint analysis provided strong evidence for presence of recombination events in majority of the sequences analyzed. However there was a clear evidence for absence or low Recombination events in viruses reported from North India. In addition we provide evidence for non-random distribution of recombination events with the highest frequency of recombination being mapped in the portion of the N-terminal .