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báo cáo hóa học:" Elevated expression of CDK4 in lung cancer"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Elevated expression of CDK4 in lung cancer | Wu et al. Journal of Translational Medicine 2011 9 38 http www.translational-medicine.eom content 9 1 38 JOURNAL OF TRANSLATIONAL MEDICINE RESEARCH Open Access Elevated expression of CDK4 in lung cancer Aibing WuH Bin Wu2t Jinsong Guo6t Weiren Luo1 Dong Wu2 Huiling Yang4 Yan Zhen1 Xiaoli Yu1 Hao Wang1 Ying Zhou1 Zhen Liu3 Weiyi Fang1 and Zhixiong Yang5 Background The aim of the present study was to analyze the expression of Cyclin-dependent kinase 4 CDK4 in lung cancer and its correlation with clinicopathologic features. Furthermore the involvement of CDK4-mediated cell cycle progression and its molecular basis were investigated in the pathogenesis of lung cancer. Methods Using immunohistochemistry analysis we analyzed CDK4 protein expression in 89 clinicopathologically characterized lung cancer patients 59 males and 30 females with ages ranging from 36 to 78 years and compared them to 23 normal lung tissues. Cases with cytoplasmic and nuclear CDK4 immunostaining score values greater than or equal to 7 were regarded as high expression while scores less than 7 were considered low expression. The correlation between the expression level of CDK4 and clinical features was analyzed. Furthermore we used lentiviral-mediated shRNA to suppress the expression of CDK4 and investigate its function and molecular mechanism for mediating cell cycle progression. Results The expression level of CDK4 protein was significantly increased in lung cancer tissues compared to normal tissues P 0.001 . In addition high levels of CDK4 protein were positively correlated with the status of pathology classification P 0.047 lymph node metastasis P 0.007 and clinical stage P 0.004 of lung cancer patients. Patients with higher CDK4 expression had a markedly shorter overall survival time than patients with low CDK4 expression. Multivariate analysis suggested the level of CDK4 expression was an independent prognostic indicator P 0.001 for the survival of patients with lung cancer. Use of .