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báo cáo hóa học:" Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài : Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus | Virology Journal BioMed Central Short report Open Access Constitutive expression of Atlantic salmon Mx1 protein in CHSE-214 cells confers resistance to Infectious Salmon Anaemia virus Molly JT Kibenge Khalid Munir and Frederick SB Kibenge Address Department of Pathology and Microbiology Atlantic Veterinary College University of Prince Edward Island 550 University Avenue Charlottetown PE. C1A 4P3. Canada Email Molly JT Kibenge - mkibenge@upei.ca Khalid Munir - kmunir@upei.ca Frederick SB Kibenge - kibenge@upei.ca Corresponding author Published 26 August 2005 Received 08 July 2005 Accepted 26 August 2005 Virology Journal 2005 2 75 doi l0.ll86 l743-422X-2-75 This article is available from http www.virologyj.cOm content 2 1 75 2005 Kibenge et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Infectious salmon anaemia ISA is a highly fatal viral disease affecting marine-farmed Atlantic salmon which is caused by ISA virus ISAV a fish orthomyxovirus that has recently been assigned to the new genus Isavirus within the family Orthomyxoviridae. Mx proteins are among the interferon IFN -induced proteins responsible for the development of an antiviral state in vertebrate cells. We used real-time reverse transcription-polymerase chain reaction RT-PCR and Chinook salmon embryo CHSE-214 cells constitutively expressing Atlantic salmon Mxl protein ASMxl to examine the antiviral properties of ASMxI against two ISAV strains NBISA01 and HKS-36 having phenotypically different growth properties cytopathic vs non-cytopathic in the CHSE-214 cell line. We present evidence that ISAV is sensitive to ASMxl. CHSE-2l4 cells constitutively expressing ASMxl showed increased resistance to infection with the cytopathic ISAV strain NBISA0l .