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báo cáo hóa học:" A phosphorylation map of the bovine papillomavirus E1 helicase"

Tuyển tập các báo cáo nghiên cứu về hóa học được đăng trên tạp chí sinh học đề tài :A phosphorylation map of the bovine papillomavirus E1 helicase | Virology Journal BioMed Central Research Open Access A phosphorylation map of the bovine papillomavirus E1 helicase Michael R Lentz 1 Stanley M Stevens Jr2 Joshua Raynes1 and Nancy Elkhoury1 Address department of Biology University of North Florida 4567 St. Johns Bluff Rd. S. Jacksonville FL 32224 USA and 2Proteomics Core Interdisciplinary Center for Biotechnology Research University of Florida Gainesville FL 32610 USA Email Michael R Lentz - mlentz@unf.edu Stanley M Stevens - sstevens@biotech.ufl.edu Joshua Raynes - neuroleo@msn.com Nancy Elkhoury - nde26@yahoo.com Corresponding author Published 08 March 2006 Received 24 August 2005 Accepted 08 March 2006 Virology Journal2006 3 13 doi 10.1186 1743-422X-3-13 This article is available from http www.virologyj.com content 3 1 13 2006Lentz et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.Org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Papillomaviruses undergo a complex life cycle requiring regulated DNA replication. The papillomavirus El helicase is essential for viral DNA replication and plays a key role in controlling viral genome copy number. The E1 helicase is regulated at least in part by protein phosphorylation however no systematic approach to phosphate site mapping has been attempted. We have utilized mass spectrometry of purified bovine papillomavirus E1 protein to identify and characterize new sites of phosphorylation. Results Mass spectrometry and in silico sequence analysis were used to identify phosphate sites on the BPV E1 protein and kinases that may recognize these sites. Five new and two previously known phosphorylation sites were identified. A phosphate site map was created and used to develop a general model for the role of phosphorylation in E1 function. Conclusion Mass .