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Báo cáo Y học: Characterization of a Saccharomyces cerevisiae NADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family

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A new NADP(H)-dependent alcohol dehydrogenase (the YCR105Wgene product, ADHVII) has been identified in Saccharomyces cerevisiae. The enzyme has been purified to homogeneity and found to be a homodimer of 40 kDa subunits and a pI of 6.2–6.4. ADHVII shows a broad sub-strate specificity similar to the recently characterized ADHVI (64% identity), although they show some differ-ences in kinetic properties. ADHVI and ADHVII are the onlymembersof the cinnamyl alcohol dehydrogenase family inyeast. SimultaneousdeletionofADH6andADH7wasnot lethal for the yeast

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