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Quantification of trans-resveratrol in Rhizoma reynoutriae japonicae by HPLC

Objectives: Establishing a HPLC method that was suitable with current conditions of the laboratory to quantification of trans-resveratrol in Rhizoma reynoutriae japonicae. Material and methods: Parameters of the established method are validated including - linear range, precision, accuracy. | JOURNAL OF MILITARY PHARMACO-MEDICINE No7-2015 QUANTIFICATION OF TRANS-RESVERATROL IN Rhizoma reynoutriae japonicae BY HPLC Hoang Van Tan*; Trinh Nam Trung**; Hoang Viet Dung** Nguyen Van Bach**; Nguyen Trong Diep** SUMMARY Objectives: Establishing a HPLC method that was suitable with current conditions of the laboratory to quantification of trans-resveratrol in Rhizoma reynoutriae japonicae. Material and methods: Parameters of the established method are validated including: linear range, precision, accuracy. Results: Chromatographic conditions of the method: using Sunfire RP-18 column, acetonitrile and 0.1% formic acid as mobile phase following a multi-step gradient program, monitored at 305 nm. The established method had the high precision (RSD = 1.65%) and high accuracy (recovery ratio = 97.32 - 102.47%, average = 99.13%, RSD = 1.14%). Conclusion: A suitable HPLC method for quantifying trans-resveratrol in Rhizoma reynoutriae japonicae was validated. * Key words: Rhizoma reynoutriae japonicae; Trans-resveratrol; HPLC. INTRODUCTION Reynoutria japonica Hautt was used to treat diseases based on traditional experience, including: treating pain of arthritis by rheumatism, swell and pain by trauma, persistant pain [6]. Information of articles indicated trans-resveratrol in Rhizoma reynoutriae japonicae possesses some interesting bioactivities, specially, the effect of modulating blood lipid status [1]. So, we selected this compound as a bioactive marker for controlling quality of medicinal plants as well as semi-products or endproducts afterwards. According to references, there were some researches of establishing HPLC methods for determinating transresveratrol together with other compounds [2, 3, 5]. However, these results were not in accordance with our research target being only to quantify the content of transresveratrol. Besides, our laboratory conditions were different from the published ones. Therefore, this research was carried out to establish HPLC .