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Genetic diversity in the natural populations of Pinus dalatensis ferré (pinaceae) assessed by SSR markers

Pinus dalatensis Ferré (Family: Pinaceae) is an endemic plant with restricted habitats at higher altitudes in highland of Vietnam. The species is now near threatened by over-exploitation and habitat destruction. The genetic variation within and among populations of P. dalatensis was investigated by 41 microsatellite (single sequence repeat, SSR) primers, but only 11 SSR primers showed polymorphism bands. In all, 70 sampled trees collected from six populations located in different altitude (Xa Hieu, Dak Glei and Ngoc Linh in Kon Tum, Da Chais in Lam Đong, Hoa Son in Dak Lak and A Yun in Gia Lai) in highland of Vietnam were analyzed in this study. | Journal of Science and Technology 54 (2) (2016) 178-189 DOI: 10.15625/0866-708X/54/2/6248 GENETIC DIVERSITY IN THE NATURAL POPULATIONS OF Pinus dalatensis Ferré (PINACEAE) ASSESSED BY SSR MARKERS Dinh Thi Phong*, Vu Thi Thu Hien, Tran Thi Lieu, Nguyen Tien Hiep Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam * Email: dinhthiphong@hotmail.com Received: 27 May 2015; Accepted for publication: 11 October 2015 ABSTRACT Pinus dalatensis Ferré (Family: Pinaceae) is an endemic plant with restricted habitats at higher altitudes in highland of Vietnam. The species is now near threatened by over-exploitation and habitat destruction. The genetic variation within and among populations of P. dalatensis was investigated by 41 microsatellite (single sequence repeat, SSR) primers, but only 11 SSR primers showed polymorphism bands. In all, 70 sampled trees collected from six populations located in different altitude (Xa Hieu, Dak Glei and Ngoc Linh in Kon Tum, Da Chais in Lam Đong, Hoa Son in Dak Lak and A Yun in Gia Lai) in highland of Vietnam were analyzed in this study. Among 40 putative genetic alleles were amplified, of which 39 were polymorphic (accounting for 97.5 %). The mean number of alleles per locus was 2.197. The SSR data showed a genetic diversity parameters within populations with an average of I = 0.524, Ho = 0.222, He = 0.317, Fis =0.333 and Ap = 0.106. The number private alleles (Ap) was only found in four populations, the Hoa Son population showed the highest (0.273), followed by Da Chais (0.182), and value of 0.091 for all Ngoc Linh and A Yun. Analysis of molecular variance analysis showed that most genetic variation was within populations of 57.504 % and among population of 42.496 %. The population pairwise differentiations indicated that most of the populations were significantly differentiated p < 0.001 with Fst values ranged from 0.195 to 0.418. This study highlights the importance