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Extraction of carotenoprotein from shrimp head using both inorganic and organic acids

Carotenoprotein was extracted by a hydrolysis method using both inorganic and organic acids. Shrimp heads were firstly hydrolyzed by adding a 0.2% (w/v) hydrochloric acid solution at room temperature for an hour following by hydrolyzing with a 0.4% (v/w) formic acid solution for another 2 h at the same temperature. The obtained carotenoprotein consisted of high contents of protein (57.8 wt.%) and carotenoids (473.9 ppm). This method is facile and efficient for the extraction of carotenoprotein from other shrimp and crab wastes on a large scale. | Journal of Fisheries science and Technology No.3 - 2016 EXTRACTION OF CAROTENOPROTEIN FROM SHRIMP HEAD USING BOTH INORGANIC AND ORGANIC ACIDS Pham Thi Dan Phuong1, Nguyen Cong Minh2, Nguyen Thi Nhu Thuong2, Nguyen Van Hoa1, Anil Kumar Anal3,Trang Si Trung1,* Received: 25/7/2016; Revised: 20/9/2016; Accepted: 26/9/2016 ABSTRACT Carotenoprotein was extracted by a hydrolysis method using both inorganic and organic acids. Shrimp heads were firstly hydrolyzed by adding a 0.2% (w/v) hydrochloric acid solution at room temperature for an hour following by hydrolyzing with a 0.4% (v/w) formic acid solution for another 2 h at the same temperature. The obtained carotenoprotein consisted of high contents of protein (57.8 wt.%) and carotenoids (473.9 ppm). This method is facile and efficient for the extraction of carotenoprotein from other shrimp and crab wastes on a large scale. Keywords: Carotenoprotein, hydrochloric acid, formic acid, shrimp head I. INTRODUCTION Carotenoid and carotenoproteins have both been considered as natural colorants and have many bioactivities such as antioxidative and antimicrobial activity (Sowmya and Sachindra, 2012; Senphan et al., 2014). Carotenoprotein has the potential to be used as a feed supplement for the pigmentation of fish flesh, and it is now being recognized as having vitamin-like qualities (Babu et al., 2008). In crustaceans, carotenoids occur as carotenoproteins, which are stable complexes of carotenoids bound to high-density lipoprotein (Shahidi et al. 1998). Many attempts have been made to recover carotenoprotein from shrimp heads using different techniques. For example, Sachindra et al. used inorganic and organic solvents to extract carotenoprotein from shrimp waste (Sachindra et al., 2006). However, the use of strong inorganic acids can degrade carotenoids and cause environmental problems. On the other hand, Cano-Lopez et al. used proteolytic enzymes to disrupt the proteincarotenoid bonds, thereby, increasing carotenoid recovery .