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Somatic embryogenesis from immature cotyledons of apomictic and non apomictic seeds in walnut (Juglans regia L.)

Somatic embryogenesis from immature cotyledons of apomictic and open-pollinated seeds in some walnut (Juglans regia L.) genotypes was investigated. | Turk J Agric For 30 (2006) 111-117 © TÜB‹TAK Somatic Embryogenesis from Immature Cotyledons of Apomictic and Non-Apomictic Seeds in Walnut (Juglans regia L.) Bekir fiAN, Hatice DUMANO⁄LU* Ankara University, Faculty of Agriculture, Department of Horticulture, 06110 Ankara - TURKEY Received: 24.03.2005 Abstract: Somatic embryogenesis from immature cotyledons of apomictic and open-pollinated seeds in some walnut (Juglans regia L.) genotypes was investigated. To obtain apomictic seeds, female flowers were bagged and/or pollinated with pollen of the apple cv. ‘Golden Delicious’ (Malus x domestica Borkh.). The best cotyledon stage for somatic embryogenesis was determined in openpollinated seeds of 10 walnut genotypes. Immature cotyledons were cultured 8, 9, 10, 11 and 12 weeks after anthesis. As a result of this experiment, cotyledons of seeds thought to be of apomictic origin were cultured 8 weeks after anthesis. Driver and Kuniyuki -1 -1 -1 walnut (DKW) medium supplemented with 1 mg l 6-benzylaminopurine (BAP), 2 mg l kinetin, 0.01 mg l indole-3-butyric acid -1 (IBA) and 250 mg l L-glutamine was used in initial cultures. Explants were transferred to DKW medium without growth regulators and L-glutamine in subcultures. The percentage of embryogenic cotyledons that originated from apomictic and non-apomictic seeds ranged from 3.6% to 25% and the number of embryos per cotyledon ranged from 1 to 9.7 at the end of the fourth subculture. A repetitively embryogenic embryo line originating from immature cotyledons of apomictic seeds of the Tokat-1 walnut genotype was maintained by secondary embryogenesis. Key Words: Walnut, Juglans regia L., somatic embryogenesis, immature cotyledon, apomictic seed Cevizde (Juglans regia L.) Apomiktik ve Apomiktik Olmayan Tohumlar›n Olgunlaflmam›fl Kotiledonlar›ndan Somatik Embriyogenesis Özet: Baz› ceviz (Juglans regia L.) genotiplerinde apomiktik ve aç›kta tozlanm›fl tohumlar›n olgunlaflmam›fl kotiledonlar›ndan somatik embriyogenesis .