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Temporal and spatial determination of germin biosynthesis in wheat tissues

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In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. | Turk J Biol 24 (2000) 775–782 © TÜBİTAK Temporal and Spatial Determination of Germin Biosynthesis in Wheat Tissues Mahmut ÇALIŞKAN, Andrew C. CUMING Mustafa Kemal University, Department of Biology, 31040, Hatay-TURKEY Received: 08.04.1999 Abstract: In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein homogenate obtained from embryos at different ages revealed higher germin accumulation than the supernatant fraction from the same sources. Key Words: Early wheat embryo development, Germin, Western blotting. Germin Biyosentezinin Buğday Dokularında Yersel ve Zamansal Tespiti Özet: Bu çalışmada germin proteininin çimlenen buğday embriyo organlarındaki biosentezi belirlenmiştir. Germin protein sentezinin yersel ve zamansal analizi germin antikor serumu kullanılarak gel-blot tekniği ile belirlenmiştir. Germin proteini 2, 3, ve 6 gün süreyle çimlendirilmiş olan buğday fidelerinin koleoriza, koleoptile ve kök bölgelerinde belirlenmiştir. Detaylı çalışmalar germin protein sentezinin yaprak dokusunda ancak çimlenmenin ileri devresinde olduğunu göstermiştir. Değişik yaşlardaki embriyolardan alınan protein homowenatları içinde pellet fraksiyonlarının supernatant fraksiyonlarına göre daha çok germin içerdiği belirlenmiştir. Anahtar Sözcükler: Erken buğday embriyo gelişimi, Germin, Western blotting. Introduction In plants embryogenesis is a developmental stage which covers the time period beginning with the formation of the zygote and ending with the formation of a mature embryo within a seed. When the mature embryo, which is a .