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Optimization of conditions for extraction of collagen from the skin of Pangasius hypophthalmus by response surface methodology

The effects of four independent variables (pepsin enzyme content, acetic-acid concentration, liquid/solid ratio, and temperature) on the extraction yield of collagen from Pangasius hypophthalmus skin were evaluated. The optimal conditions to obtain the highest yield were determinded as follows: a pepsin enzyme content of 0.25%, a acetic-acid concentration of 0.75 M, a liquid/solid ratio of 80, a temperature of 13.9 oC. The predicted yield was 73% which was in agreement with the actual value (P | Optimization of conditions for extraction of collagen from the skin of Pangasius hypophthalmus by response surface methodology Le Thi Thu Huong1 Phan Ngoc Hoa 2 Nguyen Hoang Dung 2 and Dinh Tuan 2 Phan 1 Department of Biotechnological and Environmental Engineering, Lac Hong University 2 Department of Chemical Engineering, Ho Chi Minh City University of Technology, Vietnam, 268 Ly Thuong Kiet St., Dist.10, Hochiminh City, Vietnam. Email:lethuhuong1976@yahoo.com Abstract: Collagen has been used widely in the production of pharmaceutical and cosmetic products. Fishbased collagen has still more advantages than other sources of collagen and becomes an object of interested researches recently. Extraction and some properties of collagens from the skin of Pangasius hypophthalmus fish were investigated. Pepsin enzyme and acetic-acid solution was used as an extracting solvent. The optimal conditions for collagen extraction were determined by response surface methodology. The effects of four independent variables (pepsin enzyme content, acetic-acid concentration, liquid/solid ratio, and temperature) on the extraction yield of collagen from Pangasius hypophthalmus skin were evaluated. The optimal conditions to obtain the highest yield were determinded as follows: a pepsin enzyme content of 0.25%, a acetic-acid concentration of 0.75 M, a liquid/solid ratio of 80, a temperature of 13.9 oC. The predicted yield was 73% which was in agreement with the actual value (P<0.05). The molecular weights of α1, α2 and β chains in collagen were estimated to be 130, 140 and 250 kDa, respectively. Keywords: fish collagen, pangasius hypophthalmus, optimization, response surface Tóm tắt: Collagen được sử dụng rộng rãi trong sản xuất các sản phẩm dược phẩm, mỹ phẩm. Gần đây, collagen tách chiết từ cá được ưa chuộng hơn collagen tách chiết từ các nguồn nguyên liệu khác. Bài báo này đề cập đến việc tách chiết collagen từ da cá tra Pangasius hypophthalmus và xác định một số tính chất của .