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Field and agroinoculation screening for resistance against MYMV in mungbean backcross populations

Mungbean [Vigna radiata (L.) Wilczek] known as green gram is one of the major fastgrowing, warm-season crop that is primarily cultivated in developing countries of Asia. The production of mungbean is severely constrained by mungbean yellow mosaic virus (MYMV) caused by begomo viruses, which is transmitted by whitefly, Bemesia tabaci. The absence of resistant/tolerant sources against MYMV disease leads to tremendous crop yield losses. To identify the sources of resistance in mungbean against MYMV and to utilize in further breeding programme, 115 BC2F1 lines were screened under natural conditions and categorized into different reaction groups using rating scale against MYMV. In the current study, the screened lines were also re-evaluated under green-house conditions using agroinoculation screening technique in order to confirm the sources of resistance against MYMV disease incidence. Out of them, 10 were categorized as resistant (R) in field level screening and 6 were re-confirmed through agroinoculation screening. These results can be further utilized to validate the molecular markers to facilitate markerassisted selection for the development of MYMV resistant breeding lines. | Field and agroinoculation screening for resistance against MYMV in mungbean backcross populations