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Pluripotency of spermatogonial stem cells injected into mice which were nearly killed by gamma radiation

Spermatogonial stem cells (SSCs) are pluripotent and have properties similar to embryonic stem cells. In this experiment, we collected SSCs from young mouse testis and injected it into mature female mice which were irradiated with gamma rays to bring them to a near death state. | JOURNAL OF SCIENCE OF HNUE Chemical and Biological Sci. 2012 Vol. 57 No. 8 pp. 119-127 This paper is available online at http stdb.hnue.edu.vn PLURIPOTENCY OF SPERMATOGONIAL STEM CELLS INJECTED INTO MICE WHICH WERE NEARLY KILLED BY GAMMA RADIATION Nguyen Thi Trung Thu1 Nguyen Lai Thanh2 Vu Dinh Chat2 and Dao Thi Sen1 1 Faculty of Biology Hanoi National University of Education 2 Faculty of Biology University of Science Vietnam National University Abstract. Spermatogonial stem cells SSCs are pluripotent and have properties similar to embryonic stem cells. In this experiment we collected SSCs from young mouse testis and injected it into mature female mice which were irradiated with gamma rays to bring them to a near death state. The mice were irradiated with a dose of 900R to delete marrow hematopoietic stem cells HSCs . SSCs were injected into irradiated mice to determine potential differentiation of SSCs. The result showed that SSCs could prolong the life of the mice and increase the weight of mice that survived the radiation. Upon examination we found cell division and an increase in the number of bone marrow cells. To prove the existence of transplanted SSCs in the irradiated mice we isolated the DNA of some tissues and checked for the presence of Y chromosomes in females. Those organs which most often had SSCs were the heart kidney and bone marrow the liver and brain had the least amount of SSCs. This demonstrates that introduced SSCs divided and differentiated into some types of cells which replaced depleted cells. However further study should be done to determine the potential differentiation of SSCs in irradiated animals. Keywords Spermatogonial stem cells radiated mice gamma ray. 1. Introduction Stem cell technology has improved to open the possibility for new promising medical procedures and interesting results have been obtained in the treatment of Received March 20 2012. Accepted July18 2012. Biology Subject Classification 362 196. Contact Nguyen Thi .