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Randomized DNA libraries construction tool: A new 3-bp ‘frequent cutter’ TthHB27I/ sinefungin endonuclease with chemicallyinduced specificity

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Acoustic or hydrodynamic shearing, sonication and enzymatic digestion are used to fragment DNA. However, these methods have several disadvantages, such as DNA damage, difficulties in fragmentation control, irreproducibility and under-representation of some DNA segments. The DNA fragmentation tool would be a gentle enzymatic method, offering cleavage frequency high enough to eliminate DNA fragments distribution bias and allow for easy control of partial digests. |