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Callus induction and plant regeneration in vitro from leaf explants of mycetia balansae drake

Mycetia balansae Drake, the plant distributes in Vietnam and Laos, contains sweet-tasting triterpenes and triterpene glycosides which were discovered and published the patent by Jakob and collaborators (WIPO, 2012). This plant reproduces by seed but rarely find plantlets in nature, so very difficult to develop planting for commerce. This is first time, the Mycetia balansae Drake was multiplied by using callus culture technology from leaf explants. The seedlings were served as initial explant materials. | KỈ YẾU HỘI NGHỊ SINH VIÊN NGHIÊN CỨU KHOA HỌC NĂM HỌC 2013-2014 CALLUS INDUCTION AND PLANT REGENERATION IN VITRO FROM LEAF EXPLANTS OF MYCETIA BALANSAE DRAKE Dang Thi Thao Class K60A Faculty of Biology Supervisor Assoc. Prof. Dr. Nguyen Xuan Viet and MSc. Vu Thi Bich Huyen Abstract Mycetia balansae Drake the plant distributes in Vietnam and Laos contains sweet-tasting triterpenes and triterpene glycosides which were discovered and published the patent by Jakob and collaborators WIPO 2012 . This plant reproduces by seed but rarely find plantlets in nature so very difficult to develop planting for commerce. This is first time the Mycetia balansae Drake was multiplied by using callus culture technology from leaf explants. The seedlings were served as initial explant materials. The sterilized seeds in 20 NaOCl solution for 5 min then in a solution of 10 H2O2 for 3 min showed the highest germination rate of seeds 83.33 . The maximum callus induction was recorded on MS medium supplemented with 2 mg L -1 BAP 0.2 mg L-1 NAA 0.5 mg L-1 2 4-D and the plant regeneration ratio was 53.33 in MS medium supplemented with 2 mg L -1 BAP and 1.5 mg L-1 Kinetin. The highest percentage of direct regeneration was noticed in the MS medium supplemented with 2 mg L-1 BAP 0.2 mg L-1 NAA 30 g L-1 sucrose 7g L-1 agar. The regenerated shoots were rooted in MS medium and successfully transplanted to the land and watered with 1 10 MS solution in 8 weeks the highest survival rate of plantlet was 83.63 plantlets were transplanted from September to December . Key words Callus direct regeneration Mycetia balansae Drake seed in vitro plantlet. I. INTRODUCTION Mycetia balansae Drake Ra co pua in Vietnamese was classified in Rubiaceae family and distributed in some provinces such as Vinh Phuc Tam Dao Ha Noi Ba Vi Lam Dong Lang Hanh Dong Nai Bien Hoa Gia Ray 3 . This plant has only been used traditionally in some medicines related to kindney diseases or replacing for Glycirrhiza uralensis in the .