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Ảnh hưởng của lipoprotein tỷ trọng thấp chiết xuất từ lòng đỏ trứng gà trong bảo quản lạnh (4°C) tinh dịch chó

Mục tiêu của nghiên cứu "Ảnh hưởng của lipoprotein tỷ trọng thấp chiết xuất từ lòng đỏ trứng gà trong bảo quản lạnh (4°C) tinh dịch chó" nhằm đánh giá tác động của LDL trong bảo quản lạnh tinh dịch chó. Kết quả được đánh giá qua các chỉ tiêu gồm thời gian bảo quản, hoạt lực, sức sống tuyệt đối và tính nguyên vẹn của màng acrosome tại các mức hoạt lực 0,9; 0,5 và 0,3. Mời các bạn cùng tham khảo chi tiết! | 8 Tr íng i håc Næng L m TP. Hç Ch Minh Effects of low-density lipoprotein extracted from egg yolk on preservation of chilled canine semen 4o C Van T. Nguyen Anh T. P. Nguyen Huy D. Nguyen Phuc L. G. Ha amp Khanh T. V. Doan Faculty of Animal Science and Veterinary Medicine Nong Lam University Ho Chi Minh City Vietnam ARTICLE INFO ABSTRACT Short communication Low-density lipoprotein LDL derived from hen egg yolk has been considered an important component in the storage of canine semen Received July 01 2021 however in Vietnam there have not been many studies regarding Revised September 14 2021 this topic. Therefore this study aimed to evaluate the effectiveness Accepted October 01 2021 of LDL in the preservation of canine chilled semen in Vietnam. The assessment of semen samples was based on four criteria storage time motility absolute viability and integrity of acrosomal membrane at motility rate 0.9 0.5 and 0.3. The semen samples collected were divided into three equal parts each was then diluted with three different extenders and stored at 4o C. The extenders were LDL basic extender LDL egg yolk basic extender EY and basic extender C . The storage time of canine semen was recorded from the beginning Keywords of monitoring until the motility decreased to 0.3. The storage time of the three extenders was in the order of EY gt LDL gt C with 108 h 60 h and 48 h respectively. The absolute viability of fertilizable Canine semen sperm Sa5 in the extender was EY 768 LDL 423 and C 280 Low-density lipoprotein which was significant P lt 0.001 . The percentage of viable sperm Preservation with intact acrosome membrane at motility time A 0.9 in LDL was 59.31 higher than that in EY 30.99 . In the storage period from A 0.9 to A 0.5 the acrosome loss percentage of both EY and LDL decreased equally 7.30 and 7.23 but the storage time of EY 84 h was longer than that of LDL 48 h . In conclusion the EY gave a longer storage time higher absolute viability and longer maintain percentage