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Molecular Biology Problem Solver 35

Molecular Biology Problem Solver 35. Sách được nhiều nhà khoa học có uy tín, nhiều kinh nghiệm trong nghiên cứu thực nghiệm trình bày những vấn đề thường hay phát sinh trong phòng thí nghiệm. Do vậy mà sách không trình bày các protocol hay quy trình như các sách khác, thay vào đó các tác giả sẽ trình bày các vấn đề nhằm giúp giúp đọc giả: Tự nâng cao khả năng chẩn đoán nguyên nhân khi gặp các vấn để về kỹ thuật, quy trình, hóa chất, thuốc thử trong quá trình thực nghiệm trong phòng thí. | Figure 12.1 Vapor phase concentrations of acry-lamide-water solutions 1050 acrylamide . Cytec Industries Inc. 1995. Reprinted by permission of Cytec Inc. What Are the Symptoms of Acrylamide Poisoning The initial symptoms of acrylamide poisoning on the skin are peeling of the skin at the point of contact followed by tingling and numbness in the exposed area. If exposure by any means touch ingestion inhalation continues muscular weakness difficulty maintaining balance and clumsiness in walking and in the use of the hands may develop. A large acute exposure can produce confusion disorientation slurred speech and ataxia severe loss of balance . Muscular weakness and numbness in the extremities may also follow. Anyone exposed to any form of acrylamide should be immediately examined by a medical doctor Bio-Rad Laboratories MSDS 2000 . What Is the Medical Response to Accidental Acrylamide Exposure On your skin Wash the affected skin several times with soap for at least 15 minutes under running water. In your mouth Rinse your mouth immediately with water and seek medical attention immediately. Swallowed or inhaled If swallowed do not induce vomiting. Seek medical attention immediately. If breathed in get to fresh air and seek medical attention immediately Bio-Rad Laboratories MSDS 2000 . How Can You Dispose of Excess Unusable Acrylamide Check with your institutional or local county environmental regulators for the disposal requirements in your area. The safest Electrophoresis 335 way to dispose of a small amount of liquid acrylamide is to polymerize it in the hood in a closed plastic bag set into a beaker surrounded by a very large tightly fastened plastic bag to prevent spattering as the acrylamide polymerizes. If you have more than 100 ml to dispose of contact your local environmental safety officers to determine your recommended procedure. Acrylamide solutions emit significant heat during polymerization and polymerization of large volumes of acrylamide can be explosive