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Handbook of Microbiological Media, Fourth Edition part 63

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Handbook of Microbiological Media, Fourth Edition part 63 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | E Medium for Anaerobes 615 K2HPO4.1.0g L-Asparagine.0.5g MgSO4-7H2O.0.5g NH4 2SO4.0.5g Yeast extract.0.5g CaCl2.0.1g Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure-121 C. Pour into sterile Petri dishes or leave in tubes. Use For the cultivation of Bipolaris sorghicola and Codinaea simplex. DYAA LUP Composition per liter Agar.20.0g Glucose.10.0g Yeast extract.1.0g Asparagine.0.5g K2HPO4-3H2O.0.5g MgSO4-7H2O.0.25g FeCl3 solution.0.5mL Lupine stems.variable FeCl3 Solution Composition per 10.0mL FeCl3.1.0g Preparation of FeClj Solution Add FeCl3 to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Preparation of Medium Add components except lupine stems to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute 6.0mL volumes into tubes. Cut lupine stems into 8.0cm-long pieces. Add 2-3 lupine stems per tube. Autoclave for 15 min at 15 psi pressure-121 C. Allow tubes to cool in a slanted position. Use For the cultivation of Ceratocystis coerulescens Ceratocystis microspora Coniella pulchella Phoma lini and Phoma glomerata. DYPA See Dextrose Yeast Extract Peptone E Agar m-E Agar Composition per liter Yeast extract.30.0g Agar.15.0g NaCl.15.0g Pancreatic digest of gelatin.10.0g Esculin.1.0g Nalidixic acid.0.25g NaN3.0.15g Cycloheximide.0.05g TTC solution.15.0mL pH 7.1 0.2 at 25 C Caution Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation. TTC Solution Composition per 15.0mL 2 3 5-Triphenyltetrazolium chloride.0.15g Preparation of TTC Solution Add triphenyltetrazolium chloride to distilled deionized water and bring volume to 15.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except TTC solution to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and