Đang chuẩn bị liên kết để tải về tài liệu:
Handbook of Microbiological Media, Fourth Edition part 72

Không đóng trình duyệt đến khi xuất hiện nút TẢI XUỐNG Tải xuống

Handbook of Microbiological Media, Fourth Edition part 72 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Fraser Secondary Enrichment Hi Veg Broth Base 705 Source This medium is available as a premixed powder from Oxoid Unipath. Fraser Supplement Solution Composition per 10.0mL Ferric ammonium citrate.0.5 g Acriflavine-HCl.0.25g Nalidixic acid.0.1g Ethanol.5.0mL Preparation of Fraser Supplement Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Fraser supplement solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile Fraser supplement solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use For the isolation of Listeria species from food and environmental species. Fraser Broth Half See Half Fraser Broth Fraser HiVeg Broth Base Composition per liter NaCl.20.0g Na2HPO4-2H2O.12.0g Plant extract No. 1.5.0g Plant hydrolysate.5.0g Plant peptone.5.0g Yeast extract.5.0g LiCl.3.0g KH2PO4.1.35g Esculin.1.0g Fraser supplement solution.10.0mL pH 7.2 0.2 at 25 C Source This medium without Fraser supplement solution is available as a premixed powder from HiMedia. Caution Lithium chloride is harmful. Avoid bodily contact and inhalation of vapors. On contact with skin wash with plenty of water immediately. Fraser Supplement Solution Composition per 10.0mL Ferric ammonium citrate.0.5 g Acriflavine-HCl.0.25g Nalidixic acid.0.1g Ethanol.5.0mL Preparation of Fraser Supplement Solution Add components to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except Fraser supplement solution to distilled deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add sterile Fraser supplement solution. Mix thoroughly. Aseptically .