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Handbook of Microbiological Media, Fourth Edition part 140

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Handbook of Microbiological Media, Fourth Edition part 140 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | Phenol Red Broth Base with Plant Extract No. 1 1385 Adonitol.5.0g Beef extract.1.0g Phenol Red.0.018g pH 7.4 0.2 at 25 C Source This medium is available from HiMedia. Preparation of Medium Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes with inverted Durham tubes. Autoclave for 15 min at 15 psi pressure-121 C. Use For the detection of adonitol fermenting bacteria. Phenol Red Agar Composition per liter Agar.15.0g Pancreatic digest of casein.10.0g NaCl.5.0g Phenol Red.0.018g Carbohydrate solution.20.0mL pH 7.4 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Carbohydrate Solution Composition per 20.0mL Carbohydrate.5.0-10.0g Preparation of Carbohydrate Solution Add carbohydrate to distilled deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except carbohydrate solution to distilled deionized water and bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.4 if necessary. Autoclave for 15 min at 15 psi pressure-121 C. Cool to 45 -50 C. Aseptically add 20.0mL of sterile carbohydrate solution. Pour into sterile Petri dishes or distribute into sterile tubes. Allow tubes to cool in a slanted position. Use For the determination of fermentation reactions. Bacteria that can ferment the added carbohydrate turn the medium yellow. Phenol Red Agar Composition per liter Agar.15.0g Proteose peptone No. 3.10.0g NaCl.5.0g Beef extract.1.0g Phenol Red.0.025g Carbohydrate solution.20.0mL pH 7.4 0.2 at 25 C Source This medium is available as a premixed powder from BD Diagnostic Systems. Carbohydrate Solution Composition per 20.0mL Carbohydrate.5.0-10.0g Preparation of Carbohydrate Solution Add carbohydrate to distilled deionized water and bring volume to 20.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium Add components except carbohydrate solution to distilled deionized water and bring volume to 980.0mL. .