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Handbook of Microbiological Media, Fourth Edition part 161

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Handbook of Microbiological Media, Fourth Edition part 161 is an invaluable reference for every medical, veterinary, diagnostic, and academic laboratory, and now in its fourth edition, it is even more complete. This edition carries on the tradition of CRC Press handbook excellence, listing the formulations, methods of preparation, and uses for more than 7000 microbiological media. With 1500 more entries than any previous edition, the handbook includes both classic and modern media used for the identification, cultivation, and maintenance of diverse bacteria, archaea, and fungi | SP 4 Medium 1595 L-Serine.0.025g L-Valine.0.025g L-Cystine.0.02g L-Histidine-HCl-H2O.0.02g L-Isoleucine.0.02g Phenol Red.0.02g L-Methionine.0.015g Deoxyadenosine.0.01g Deoxycytidine.0.01g Deoxyguanosine. 0.01g Glutathione reduced.0.01g Thymidine.0.01g Hydroxy-L-proline.0.01g L-Tryptophan.0.01g Nicotinamide adenine dinucleotide.7.0mg Tween 80.5.0mg Sodium glucoronate-H2O.4.2mg Coenzyme A.2.5mg Cocarboxylase.1.0mg Flavin adenine dinucleotide.1.0mg Nicotinamide adenine dinucleotide phosphate.1.0mg Uridine triphosphate.1.0mg Choline chloride.0.5mg Cholesterol.0.2mg 5-Methyldeoxycytidine.0.1mg Inositol.0.05mg p-Aminobenzoic acid.0.05mg Niacin.0.025mg Niacinamide.0.025mg Pyridoxine.0.025mg Pyridoxal-HCl.0.025mg Biotin.0.01mg D-Calcium pantothenate.0.01mg Folic acid.0.01mg Riboflavin.0.01mg Thiamine-HCl.0.01mg Preparation of CMRL 1066 10X with Glutamine Add components to distilled deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Filter sterilize. Fresh Yeast Extract Solution Composition per 100.0mL Baker s yeast live pressed starch-free .25.0g Preparation of Fresh Yeast Extract Solution Add the live Baker s yeast to 100.0mL of distilled deionized water. Autoclave for 90 min at 15 psi pressure-121 C. Allow to stand. Remove supernatant solution. Adjust pH to 6.6-6.8. Penicillin Solution Composition per 10.0mL Penicillin G. 1 000 000U Preparation of Penicillin Solution Add penicillin G to distilled deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium To 615.0mL of cooled sterile base solution aseptically add 170.0mL of sterile inactivated fetal calf serum 100.0mL of sterile yeast extract 50.0mL of sterile CMRL 1066 10X with glutamine 35.0mL of sterile fresh yeast extract solution 20.0mL of Phenol Red solution and 10.0mL of sterile penicillin solution. Mix thoroughly. Aseptically distribute into sterile tubes. Allow tubes to cool in a slanted position. Use For the cultivation of tick-derived Mycoplasma

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