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Báo cáo lâm nghiệp: "Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí lâm nghiệp đề tài: Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants. | JOURNAL OF FOREST SCIENCE 57 2011 7 277-280 Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants D. Pavingerová J. Briza H. Niedermeierová J. Vlasák Institute of Plant Molecular Biology Biology Centre of the Academy of Sciences of the Czech Republic Ceské Budễịovice Czech Republic ABSTRACT In conifers and other plants with long reproductive cycles transformed embryogenic tissues can serve as a convenient source of plant material for the testing of insecticidal or fungicidal transgene efficiency. In this report transgenic embryogenic tissue was obtained after the transformation of somatic embryos of Norway spruce Picea abies L. Karst. by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only in a suitable embryogenic cell line sensitive to Agrobacterium. Out of the nine embryogenic lines tested only one gave transgenic callus. Keywords Agrobacterium tumefaciens genetic engineering GUS activity Picea abies L. Karst. Conventional plant breeding methods have resulted in significant genetic gains in some conifers Shelbourne et al. 1989 . The long reproductive cycles of conifers however render conventional breeding techniques highly time consuming and some desirable traits of commercial value such as insect and fungal resistance are not available in the breeding populations. The genetic engineering methods and tissue culture technologies offer faster and more efficient introduction of desired attributes. Genetic transformation of plants by Agrobacterium tumefaciens is the preferred method of transgene integration into plant genome. A stable transformation procedure has been developed also for various forest tree species e.g. Bajaj 2000 the first transgenic tree was described in 1987 Fillatti et al. 1987 . Transgenic conifers were reported about 15 years ago Huang et al. 1991 and to .