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Báo cáo khoa học: "Development of immunoassays for the detection of kanamycin in veterinary fields"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Development of immunoassays for the detection of kanamycin in veterinary fields | J. Vet. Set. 2006 7 2 111-117 JOURNAL OF Veterinary Science Development of immunoassays for the detection of kanamycin in veterinary fields Yong Jin1 Jin-Wook Jang2 Chang-Hoon Han1 Mun-Han Lee2 institute for Zoonotic Disease College of Veterinary Medicine Seoul National University Seoul 151-742 Korea department of Biochemistry College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Monoclonal antibody against kanamycin was prepared and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgGl which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA the detection limits of kanamycin were determined to be 1.1 ng ml in PBS 1.4 ng ml in plasma and 1.0 ng ml in milk. The concentration of intramuscularly injected kanamycin was successfully monitored in rabbit plasma with competitive direct ELISA. Based on the colloidal gold-based immunochromatographic assay the detection limits of kanamycin were estimated to be about 6-8 ng ml in PBS plasma and milk. The immunochromatographic assay would be suitable for rapid and simple screening of kanamycin residues in veterinary medicine. Screened positives can be confirmed using a more sensitive laboratory method such as competitive direct ELISA. Therefore the assays developed in this study could be used to complement each other as well as other laboratory findings. Moreover instead of slaughtering the animals to obtain test samples these methods could be applied to determine kanamycin concentration in the plasma of live animals. Key words competitive direct ELISA immunochromatographic assay kanamycin monoclonal antibody Introduction Kanamycin an aminoglycoside antibiotic produced by Streptomyces kanamyceticus is widely