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Báo cáo khoa học: "Phylogenetic characterization of genes encoding for glycoprotein 5 and membrane protein of PRRSV isolate HH08"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Phylogenetic characterization of genes encoding for glycoprotein 5 and membrane protein of PRRSV isolate HH08 | J. Vet. Sci. 2009 10 4 309-315 DOI 10.4142 jvs.2009.10.4.309 JOURNAL OF Veterinary Science Phylogenetic characterization of genes encoding for glycoprotein 5 and membrane protein of PRRSV isolate HH08 Mingcui Wang1 Guangxing Li1 Jiechao Yin2 Xiaofeng Ren1 College ofVeterinary Medicine and 2College of Life Sciences Northeast Agricultural University 59 Mucai Street Xiangfang District 150030 Harbin China A porcine reproductive and respiratory syndrome virus PRRSV was obtained from clinic samples. Genes 5 and 6 encoding for the viral glycoprotein 5 and a membrane protein of the PRRSV designated as HH08 were amplified by reverse transcription-PCR. These sequences were compared with reference sequences derived from different geographical locations. The results indicated that the virus belongs to the North American type rather than European. Comparative analyses of the genetic diversity between the PRRSV isolate HH08 and other Chinese as well as foreign reference strains of PRRSV were discussed based on the sequence comparison and the topology of phylogenetic trees constructed in this study. Keywords GP5 M protein phylogenetic analysis PRRSV Introduction Porcine reproductive and respiratory syndrome virus PRRSV is the causative agent of PRRS. This disease is an emerging swine disease that was originally recognized in North America in 1987 and in Europe in 1990 8 18 . Since the first PRRSV was isolated in Europe in 1990 the disease became one of the most economically important diseases in most pig-producing countries 5 13 . PRRSV is an enveloped single-stranded positive sense RNA virus belonging to the family of Arteriviridae order Nidovirales 2 . The approximately 15 kb viral genome encompasses nine identified open reading frames ORFs . ORFs 1a and 1b encode viral replicase polyproteins and ORFs 2a 2b 3 through 7 encode the viral structural proteins glycoprotein GP 2 envelope E GP3 GP4 GP5 membrane M protein as well nucleocapsid N protein respectively 16 . The North .