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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

Tuyển tập các báo cáo nghiên cứu khoa học ngành y học tạp chí Medical Sciences dành cho các bạn sinh viên ngành y tham khảo đề tài: Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study. | Int. J. Med. Sci. 2011 8 222 International Journal of Medical Sciences 2011 8 3 222-230 Research Paper Characterization of Human Erythrocytes as Potential Carrier for Pravastatin An In Vitro Study Gamal El-din I. Harisa Mohamed F. Ibrahim Fars K. Alanazi x Department of Pharmaceutics College of Pharmacy King Saud University P.O. Box 2457 Riyadh 11451 Saudi Arabia H Corresponding author Fars K. Alanazi Kayyali Chair for Pharmaceutical Industry. afars@ksu.edu.sa Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License http creativecommons.org licenses by-nc-nd 3.0 . Reproduction is permitted for personal noncommercial use provided that the article is in whole unmodified and properly cited. Received 2011.01.04 Accepted 2011.02.18 Published 2011.03.11 Abstract Drug delivery systems including chemical physical and biological agents that enhance the bioavailability improve pharmacokinetics and reduce toxicities of the drugs. Carrier erythrocytes are one of the most promising biological drug delivery systems investigated in recent decades. The bioavailability of statin drugs is low due the effects of P-glycoprotein in the gastro-intestinal tract as well as the first-pass metabolism. Therefore in this work we study the effect of time temperature as well as concentration on the loading of pravastatin in human erythrocytes to be using them as systemic sustained release delivery system for this drug. After the loading process is performed the carriers erythrocytes were physically and cellulary characterized. Also the in vitro release of pravastatin from carrier erythrocytes was studied over time interval. Our results revealed that human erythrocytes have been successfully loaded with pravastatin using endocytosis method either at 25oC or at 37oC. The loaded amount at 10 mg ml is 0.32mg 0.1 ml and 0.69 mg 0.1 ml. Entrapment efficiency is 34 and 94 at 25oC and 37oC respectively at drug concentration 4 mg ml. .