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Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin. | Available online http arthritis-research.eom content 7 1 R47 Open Access Research article The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin Ian Giles1 2 Nancy Lambrianides1 2 David Latchman2 Pojen Chen3 Reginald Chukwuocha3 David Isenberg1 and Anisur Rahman1 2 1Centre for Rheumatology Department of Medicine University College London UK 2Medical Molecular Biology Unit Institute of Child Health University College London UK 3Department of Medicine Division of Rheumatology University of California Los Angeles USA Corresponding author Ian Giles i.giles@ich.ucl.ac.uk Received 28 May 2004 Revisions requested 10 Aug 2004 Revisions received 31 Aug 2004 Accepted 23 Sep 2004 Published 16 Nov 2004 Arthritis Res Ther 2005 7 R47-R56 DOI 10.1186 ar1449 2004 Giles et al. licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is cited. Abstract Previously we reported that the variable heavy chain region VH of a human beta2 glycoprotein I-dependent monoclonal antiphospholipid antibody IS4 was dominant in conferring the ability to bind cardiolipin CL . In contrast the identity of the paired variable light chain region VL determined the strength of CL binding. In the present study we examine the importance of specific arginine residues in IS4VH and paired VL in CL binding. The distribution of arginine residues in complementarity determining regions CDRs of VH and VL sequences was altered by site-directed mutagenesis or by CDR exchange. Ten different 2a2 germline gene-derived VL sequences were expressed with IS4VH and the VH of an anti-dsDNA antibody B3. Six variants of IS4VH containing different patterns of arginine residues in CDR3 were paired with B3VL and IS4VL. The ability Keywords antiphospholipid antibodies .