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Báo cáo y học: "Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài:"Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells. | Wemhoner et al. Respiratory Research 2010 11 52 http respiratory-research.eom content 11 1 52 RESPIRATORY RESEARCH RESEARCH Open Access Effects of Perfluorocarbons on surfactant exocytosis and membrane properties in isolated alveolar type II cells Andreas Wemhoner 1 2 Irmgard Hackspiel2 Nina Hobi3 Andrea Ravasio3 Thomas Haller3 and Mario Rudiger 1 2 Abstract Background Perfluorocarbons PFC are used to improve gas exchange in diseased lungs. PFC have been shown to affect various cell types. Thus effects on alveolar type II ATII cells and surfactant metabolism can be expected data however are controversial. Objective The study was performed to test two hypotheses I the effects of PFC on surfactant exocytosis depend on their respective vapor pressures II different pathways of surfactant exocytosis are affected differently by PFC. Methods Isolated ATII cells were exposed to two PFC with different vapor pressures and spontaneous surfactant exocytosis was measured. Furthermore surfactant exocytosis was stimulated by either ATP PMA or Ionomycin. The effects of PFC on cell morphology cellular viability endocytosis membrane permeability and fluidity were determined. Results The spontaneous exocytosis was reduced by PFC however the ATP and PMA stimulated exocytosis was slightly increased by PFC with high vapor pressure. In contrast Ionomycin-induced exocytosis was decreased by PFC with low vapor pressure. Cellular uptake of FM 1-43 - a marker of membrane integrity - was increased. However membrane fluidity endocytosis and viability were not affected by PFC incubation. Conclusions We conclude that PFC effects can be explained by modest unspecific interactions with the plasma membrane rather than by specific interactions with intracellular targets. Introduction Perfluorocarbons PFC that are used to improve gas exchange in diseased lungs with disturbed pulmonary surfactant system affect various cell types 1-4 . Thus an effect on alveolar type II ATII cells and surfactant .