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Báo cáo khoa học: "Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs"

Tuyển tập các báo cáo nghiên cứu về bệnh thú y được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành thú y đề tài: Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs. | Acta vet. scand. 2001 42 279-286. Optimization of the Agar-gel Method for Isolation of Migrating Ascaris suum Larvae From the Liver and Lungs of Pigs By I. Saeed1 A. Roepstorff1 T Rasmussen1 2 M. Hag1 and G. Jungersen2 3 1Danish Centre For Experimental Parasitology 2Department of Clinical Studies The Royal Veterinary and Agricultural University Frederiksberg and 3Danish Veterinary Laboratory Copenhagen Denmark. Saeed I Roepstorff A Rasmussen T Hog M Jungersen G Optimization of the agar-gel method for isolation of migrating Ascaris suum larvae from the liver and lungs of pigs. Acta vet. scand. 2001 42 279-286. - Experiments on use of an agar-gel method for recovery of migrating Ascaris suum larvae from the liver and lungs of pigs were conducted to obtain fast standardized methods. Subsamples of blended tissues of pig liver and lungs were mixed with agar to a final concentration of 1 agar and the larvae allowed to migrate out of the agar-gel into 0.9 NaCl at 38 C. The results showed that within 3 h more than 88 of the recoverable larvae migrated out of the liver agargel and more than 83 of the obtained larvae migrated out of the lung agar-gel. The larvae were subsequently available in a very clean suspension which reduced the sample counting time. Blending the liver for 60 sec in a commercial blender showed significantly higher larvae recovery than blending for 30 sec. Addition of gentamycin to reduce bacterial growth during incubation glucose to increase larval motility during migration or ice to increase sedimentation of migrated larvae did not influence larvae recovery significantly. Ascaris suum larva recovery agar-gel method liver lungs. Introduction Simple microscopical quantification of Ascaris suum larvae migrating in the liver and lungs of pigs as carried out by e.g. Dourvres et al. 1969 is very labourous while the use of a modified Baermann technique on blended tissue Eriksen et al. 1992 has been shown to reduce the work-load considerably. Jargensen 1975 .