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Báo cáo sinh học: "Comparative diffusion assay to assess efficacy of topical antimicrobial agents against Pseudomonas aeruginosa in burns care"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí y học Molecular Biology cung cấp cho các bạn kiến thức về ngành sinh học đề tài: Comparative diffusion assay to assess efficacy of topical antimicrobial agents against Pseudomonas aeruginosa in burns care. | Aujoulat et al. Annals of Clinical Microbiology and Antimicrobials 2011 10 27 http www.ann-clinmicrob.eom content 10 1 27 ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS RESEARCH Open Access Comparative diffusion assay to assess efficacy of topical antimicrobial agents against Pseudomonas aeruginosa in burns care 1 2 1.3 1 14 2 Fabien Aujoulat Frangoise Lebreton Sara Romano Milena Delage Hélène Marchandin Monique Brabet Frangoise Bricard3 Sylvain Godreuil4 Sylvie Parer3 and Estelle Jumas-Bilak1 3 Abstract Background Severely burned patients may develop life-threatening nosocomial infections due to Pseudomonas aeruginosa which can exhibit a high-level of resistance to antimicrobial drugs and has a propensity to cause nosocomial outbreaks. Antiseptic and topical antimicrobial compounds constitute major resources for burns care but in vitro testing of their activity is not performed in practice. Results In our burn unit a P. aeruginosa clone multiresistant to antibiotics colonized or infected 26 patients over a 2-year period. This resident clone was characterized by PCR based on ERIC sequences. We investigated the susceptibility of the resident clone to silver sulphadiazine and to the main topical antimicrobial agents currently used in the burn unit. We proposed an optimized diffusion assay used for comparative analysis of P. aeruginosa strains. The resident clone displayed lower susceptibility to silver sulphadiazine and cerium silver sulphadiazine than strains unrelated to the resident clone in the unit or unrelated to the burn unit. Conclusions The diffusion assay developed herein detects differences in behaviour against antimicrobials between tested strains and a reference population. The method could be proposed for use in semi-routine practice of medical microbiology. Keywords Pseudomonas aeruginosa burns silver sulphadiazine antiseptics ERIC-PCR diffusion assay Background The current techniques of resuscitation surgery and wound care have significantly .