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Báo cáo y học: "Hyaluronan modulates accumulation of hypoxiainducible factor-1 alpha, inducible nitric oxide synthase, and matrix metalloproteinase-3 in the synovium of rat adjuvant-induced arthritis model"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Hyaluronan modulates accumulation of hypoxiainducible factor-1 alpha, inducible nitric oxide synthase, and matrix metalloproteinase-3 in the synovium of rat adjuvant-induced arthritis model. | Chou et al. Arthritis Research Therapy 2011 13 R90 http arthritis-research.eom content 13 3 R90 RESEARCH ARTICLE Open Access Hyaluronan modulates accumulation of hypoxiainducible factor-1 alpha inducible nitric oxide synthase and matrix metalloproteinase-3 in the synovium of rat adjuvant-induced arthritis model Li-Wei Chou1 2 3t John Wang4 5t Pei-Lin Chang1 and Yueh-Ling Hsieh1 Abstract Introduction Hypoxia is a feature of the inflamed synovium in rheumatoid arthritis RA . Intra-articular injection of hyaluronan HA may be considered a potential way to treat RA. However the exact molecular mechanism of HA on decreased cellular responses to hypoxic environment is unclear. The present study has been designed to use the adjuvant-induced arthritis model to examine the effects of HA on the changes of immunohistochemical expressions of hypoxia-inducible factor-1alpha HIF-1alpha inducible nitric oxide synthase iNOS and matrix metalloproteinase-3 MMP3 in the synovial tissues at the early phase of arthritic inflammation. Methods Monoarthritis was induced in adult male Sprague-Dawley 250-300 g via intraarticular injection of complete Freund s adjuvant CFA into the tibiotarsal joint. The CFA-induction arthritis animals were divided into three groups treatment intraarticular injection of HA placebo intraarticular injection of saline and controls no treatments . Functional evaluations of edema and pain behavior histology and HIF-1alpha iNOS and MMP3 immunohistochemistry were performed before after the first injection three injections and on the follow-up injection of the treatments. Results Intra-articular injection of HA also significantly suppressed the mechanical allodynia p 0.001 and overexpressions of HIF-1alpha p 0.001 iNOS p 0.004 and MMP3 p 0.001 immunoreactivity in synovium. Conclusions This study demonstrated that early intervention of HA is an effective protection against accumulation of inflammation-induced HIF-1alpha iNOS and MMP3 to limit erosive damage in .