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Báo cáo y học: "Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú y đề tài: Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia. | Sharif et al. Acta Veterinaria Scandinavica 2010 52 1 http www.actavetscand.eom content 52 1 1 AVS ACTAVETERINARIA SCANDINAVICA BRIEF COMMUNICATION Open Access Descriptive distribution and phylogenetic analysis of feline infectious peritonitis virus isolates of Malaysia Saeed Sharif1 Siti S Arshad1 Mohd Hair-Bejo1 Abdul R Omar1 Nazariah A Zeenathul1 Lau S Fong2 Nor-Alimah Rahman3 Habibah Arshad2 Shahirudin Shamsudin2 Mohd-Kamarudin A Isa1 Abstract The descriptive distribution and phylogeny of feline coronaviruses FCoVs were studied in cats suspected of having feline infectious peritonitis FIP in Malaysia. Ascitic fluids and or biopsy samples were subjected to a reverse transcription polymerase chain reaction RT-PCR targeted for a conserved region of 3 untranslated region 3 UTR of the FCoV genome. Eighty nine percent of the sampled animals were positive for the presence of FCoV. Among the FCoV positive cats 80 of cats were males and 64 were below 2 years of age. The FCoV positive cases included 56 domestic short hair DSH 40 Persian and 4 Siamese cats. The nucleotide sequences of 10 selected amplified products from FIP cases were determined. The sequence comparison revealed that the field isolates had 96 homology with a few point mutations. The extent of homology decreased to 93 when compared with reference strains. The overall branching pattern of phylogenetic tree showed two distinct clusters where all Malaysian isolates fall into one main genetic cluster. These findings provided the first genetic information of FCoV in Malaysia. Findings Feline infectious peritonitis FIP is a highly fatal disease of cats caused by generalized infection with a feline coronavirus FCoV . FCoVs belong to subgroup 1a of Coronaviruses in the family Coronaviridae order Nidovirales. Other members of this subgroup include porcine transmissible gastroenteritis virus canine coronavirus rac-coon dog coronavirus and Chinese ferret badger coronavirus 1 2 . FCoVs are enveloped positive-strand .