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Báo cáo khoa học: "Plasma creatinine in dogs: intra- and interlaboratory variation in 10 European veterinary laboratories"

Tuyển tập các báo cáo nghiên cứu về bệnh học thý y được đăng trên tạp chí Acta Veterinaria Scandinavica cung cấp cho các bạn kiến thức về bệnh thú yđề tài: Plasma creatinine in dogs: intra- and interlaboratory variation in 10 European veterinary laboratories. | Ulleberg et al. Acta Veterinaria Scandinavica 2011 53 25 http www.actavetscand.eom content 53 1 25 AVS ACTAVETERINARIA SCANDINAVICA RESEARCH Open Access Plasma creatinine in dogs intra- and interlaboratory variation in 10 European veterinary laboratories Tina Ulleberg1 Joris Robben2 Kathrine M Nordahl3 Thomas Ulleberg4 and Reidun Heiene5 Abstract Background There is substantial variation in reported reference intervals for canine plasma creatinine among veterinary laboratories thereby influencing the clinical assessment of analytical results. The aims of the study was to determine the inter- and intra-laboratory variation in plasma creatinine among 10 veterinary laboratories and to compare results from each laboratory with the upper limit of its reference interval. Methods Samples were collected from 10 healthy dogs 10 dogs with expected intermediate plasma creatinine concentrations and 10 dogs with azotemia. Overlap was observed for the first two groups. The 30 samples were divided into 3 batches and shipped in random order by postal delivery for plasma creatinine determination. Statistical testing was performed in accordance with ISO standard methodology. Results Inter- and intra-laboratory variation was clinically acceptable as plasma creatinine values for most samples were usually of the same magnitude. A few extreme outliers caused three laboratories to fail statistical testing for consistency. Laboratory sample means above or below the overall sample mean did not unequivocally reflect high or low reference intervals in that laboratory. Conclusions In spite of close analytical results further standardization among laboratories is warranted. The discrepant reference intervals seem to largely reflect different populations used in establishing the reference intervals rather than analytical variation due to different laboratory methods. Introduction Creatinine is produced in muscle at a constant rate and excreted by the kidney primarily through glomerular .