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Báo cáo y học: " Association between disruption of CD4 receptor dimerization and increased human immunodeficiency virus type 1 entry"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: " Association between disruption of CD4 receptor dimerization and increased human immunodeficiency virus type 1 entry. | Retrovirology BioMed Central Research Open Access Association between disruption of CD4 receptor dimerization and increased human immunodeficiency virus type 1 entry Rachel Bourgeois1 Johanne Mercier2 Isabelle Paquette-Brooks2 and Éric A Cohen 1 Address Department of Microbiology and Immunology Université de Montréal Montreal Quebec Canada and 2Laboratory of Human Retrovirology Institut de Recherches Cliniques de Montréal Montreal Quebec Canada Email Rachel Bourgeois - rachel.bourgeois@umontreal.ca Johanne Mercier - johanne.mercier@ircm.qc.ca Isabelle Paquette- Brooks - dauphin1492@hotmail.com Éric A Cohen - eric.cohen@ircm.qc.ca Corresponding author Published 08 June 2006 Received 24 April 2006 Retrovirology 2006 3 31 doi 10.1186 1742-4690-3-3 1 Accepted 08 June 2006 This article is available from http www.retrovirology.cOm content 3 1 3 1 2006 Bourgeois et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http creativecommons.org licenses by 2.0 which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Human immunodeficiency virus HIV enters target cells by a membrane fusion process that involves a series of sequential interactions between its envelope glycoproteins the CD4 receptor and CXCR4 CCR5 coreceptors. CD4 molecules are expressed at the cell surface of lymphocytes and monocytes mainly as monomers but basal levels of CD4 dimers are also present at the cell surface of these cells. Previous evidence indicates that the membrane distal and proximal extracellular domains of CD4 respectively D1 and D4 are involved in receptor dimerization. Results Here we have used A201 cell lines expressing two CD4 mutants CD4-E91 K E92K D1 mutant and CD4-Q344E D4 mutant harboring dimerization defects to analyze the role of CD4 dimerization in HIV-1 entry. Using entry assays based on P-lactamase-Vpr or luciferase